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KOMP Repository (Knockout Mouse Project) KOMP Knock Out Knockout Mouse Mice
ProtocolCSDVelocigene
Culturing and media conditions for KOMP CSD JM8 N4 ES cell clones CSD
Culturing and media conditions for KOMP CSD JM8A (Agouti) ES cell clones CSD
Culturing and media conditions for KOMP CSD JM8 and JM8 F6 ES cell clones CSD
Culturing and Media conditions for KOMP Velocigene VGB6 ES cell clones Velocigene
Chromosome Spreads and Counting KOMP ES cell clones CSDVelocigene
Genotyping of KOMP CSD targeted alleles CSD
Long Range PCR protocol for KOMP CSD targeted alleles CSD
Genotyping of KOMP Velocigene targeted alleles Velocigene
Loss of Native Allele for KOMP Velocigene targeted alleles Velocigene
Microinjection of blastocysts for CSD JM8A clones CSD
Microinjection of morulae for KOMP Velocigene VGB6 ES cell clones Velocigene
Microinjection of blastocysts for JM8 and VGB6 clones CSDVelocigene
Germline Testing of KOMP C57BL/6N (non-agouti) clones CSDVelocigene
Germline Testing of KOMP JM8A (agouti) clones CSD
Electroporation of JM8 Cells and Selection of Targeted Clones CSD
Electroporation into JM8 cells with FlpE plasmid CSD
Electroporation into JM8 cells with Cre plasmid CSD
EAP1 Culture Protocol
Loss of Native Allele for KOMP CSD targeted alleles (currently unavailable) CSD


The KOMP Repository is located at the University of California Davis and Children’s Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at service@komp.org, US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors komporders@chori.org or +1-510-450-7917.