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KOMP Repository (Knockout Mouse Project)

KOMP Knock Out Knockout Mouse Mice

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KOMP Repository Product Detail Sheet

Target Gene Details

Symbol:	Tgfb1
Name:	transforming growth factor, beta 1
MGI:	MGI:98725
Chromosome:	7

Project Details

Targeting Project:	CSD47880
Alleles:	Tgfb1^{tm1a(KOMP)Wtsi}, Tgfb1^{tm1e(KOMP)Wtsi}
Mutations:	Knockout First (Promoter driven), Targeted, non-conditional (Promoter driven)
Parental Cell Lines:	JM8A1.N3
Strains:	C57BL/6N-A^tm1Brd
Coat Colors:	Agouti (A/a)

Applicable Protocols

Chromosome Spreads and Counting KOMP ES cell clones
Long Range PCR protocol for KOMP CSD targeted alleles
Genotyping of KOMP CSD targeted alleles
Electroporation into JM8 cells with FlpE plasmid
Electroporation into JM8 cells with Cre plasmid
Germline Testing of KOMP JM8A (agouti) clones
Microinjection of blastocysts for CSD JM8A clones
Culturing and media conditions for KOMP CSD JM8A (Agouti) ES cell clones

Applicable Material Transfer Agreements (MTAs)

KOMP Targeted Products (Vectors, ES cells, Mice, Germplasm)
KOMP Collaborator MTA

The KOMP Repository Collection is located at the MMRRC at the University of California, Davis and Children’s Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at mmrrc@ucdavis.edu, US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors komporders@chori.org or +1-510-450-7917.