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KOMP Repository (Knockout Mouse Project) KOMP Knock Out Knockout Mouse Mice
KOMP Repository Product Detail Sheet
Cell Line Details
Cell Line: JM8A3 subline
Project: PL236747
Mutation: Wildtype
Strain: C57BL/6N-Atm1Brd
Coat Color: Agouti (A/a)
Origin: Sub-cloned from JM8 parental cell line, which was derived from C57BL/6N mice. These ES cells have been modified to correct the black mutation on the Agouti allele, and mice derived from these cells will have an Agouti coat color. See publication: Pettitt et al.: Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nature Methods, 2009

Additional Information: The KOMP Repository has recently identified the presence of a translocation of the manipulated agouti allele on chromosome 8 of ES cell line JM8A3, and potentially affecting the sub-lines JM8A3.N1 and JM8A1.N3, that were all derived at the Wellcome Trust Sanger Institute. At this time, we recommended not to use these cells for targeting genes on chromosome 8. Other lines, including, the JM8, JM8.N4, or JM8.F6 , do not exhibit this translocation and can be safely and reliably used for gene targeting experiments. If you are using mouse lines (e.g., KOMP targeted lines) derived from JM8A3, JM8A3.N1 or JM8A1.N3 ES cell lines, we recommend crossing to C57BL/6N wild type mice in order to breed out the agouti coat color. For further information or assistance please contact Allan Bradley at The Sanger Institute abradley@sanger.ac.uk.
Availability:

Cell Line Images

JM8A3 p10
Plated on 6 well with Gelatin in M15
Cells ready for passage
4x Light Microscopy
Photo taken 12/14/2009 by TM
View full size
JM8A3 p10
Plated on 6 well with Gelatin in M15
Cells ready for passage
4x Light Microscopy
Photo taken 12/14/2009 by TM
View full size
JM8A3 p11
Plated on 10cm with Gelatin in M15
Cells passed 12-14 hours prior; fed 4 hours prior to photograph
4x Light Microscopy
Photo taken 12/15/2009 by TM
View full size

Applicable Protocols
Applicable Material Transfer Agreements (MTAs)


The KOMP Repository Collection is located at the MMRRC at the University of California, Davis and Children’s Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at mmrrc@ucdavis.edu, US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors komporders@chori.org or +1-510-450-7917.