KOMP Knock Out Knockout Mouse Mice|
Overview of throughput of KOMP products in the KOMP Repository
October 4, 2007
Approximately every two months, the KOMP Repository acquires vectors, ES cells, and mice from the two KOMP
mutagenesis teams, the CSD Consortium (CHORI, Sanger Institute, and UC Davis) and Regeneron.
including targeted ES cell clones, clone database, targeting vectors, mice, embryos/sperm are then processed
in the following manner:
- All KOMP ES clones received are recorded and archived upon receipt.
Once archived, one viable clone from each successfully targeted gene is selected according
to \“best clone\” data available from the mutagenesis teams.
These selected clones are expanded into ~12 vials each for quality control (QC) testing,
including viability-growth-morphology and pathogen assessment. 5% of selected clones
undergo genotype verification and chromosome counting. Once these clones pass in vitro
testing, they are then archived and released for distribution to requesting investigators.
The same 5% clones are then processed for in vivo testing, which includes microinjection to
produce high percentage chimeras, germline transmission testing, and cre/flp recombination
of the targeted allele (on 1% of CSD produced clones only). Germline-transmitted mice,
embryos, and sperm generated are then archived and released for distribution.
- All KOMP targeting vectors received (in glycerol stocks) will be sent to CHORI for QC,
archiving, and distribution. A small percentage of vectors will be re-sequenced and
transformed. Once targeting vectors pass QC at CHORI, they will then be archived and
released for distribution.
- All KOMP mice, embryos, and sperm received from the mutagenesis teams will also be
rederived and used to create a cryopreserved embryo and sperm archive which will
subsequently be released for distribution.
- All KOMP-related data received in association with products from the KOMP mutagenesis
teams is screened for errors and scrubbed prior to posting on our website
(www.komp.org) catalog, list of protocols,
\“product detail files\” (PDF), etc.
The KOMP Repository is the sole archive and distribution repository for vectors, ES cells, and mice
generated by the KOMP mutagenesis centers. Supported by an NIH cooperative agreement (U42 mechanism) to the
University of California, Davis (UCD) and its subcontractor, Children's Hospital Oakland Research Institute
(CHORI), the KOMP Repository ensures the preservation, protection, availability, and accessibility of KOMP
resources for use by the scientific community.
The repository is upon the closely linked infrastructure, technical support, and faculty expertise within
the UCD Mouse Biology Program, Center for Comparative Medicine, the Schools of Medicine and Veterinary
Medicine, and the CHORI-BACPAC Resource.
UCD archives, maintains, and distribute ES cell clones, live mouse lines, and frozen embryos and sperm,
while CHORI archives, maintains, and distribute all vectors.
The KOMP Repository is located at the University of California Davis and Children’s Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at firstname.lastname@example.org, US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors email@example.com or +1-510-450-7917.