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KOMP Repository (Knockout Mouse Project) KOMP Knock Out Knockout Mouse Mice
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Overview of throughput of KOMP products in the KOMP Repository

October 4, 2007

Approximately every two months, the KOMP Repository acquires vectors, ES cells, and mice from the two KOMP mutagenesis teams, the CSD Consortium (CHORI, Sanger Institute, and UC Davis) and Regeneron. All \“products\”, including targeted ES cell clones, clone database, targeting vectors, mice, embryos/sperm are then processed in the following manner:
  • All KOMP ES clones received are recorded and archived upon receipt. Once archived, one viable clone from each successfully targeted gene is selected according to \“best clone\” data available from the mutagenesis teams. These selected clones are expanded into ~12 vials each for quality control (QC) testing, including viability-growth-morphology and pathogen assessment. 5% of selected clones undergo genotype verification and chromosome counting. Once these clones pass in vitro testing, they are then archived and released for distribution to requesting investigators. The same 5% clones are then processed for in vivo testing, which includes microinjection to produce high percentage chimeras, germline transmission testing, and cre/flp recombination of the targeted allele (on 1% of CSD produced clones only). Germline-transmitted mice, embryos, and sperm generated are then archived and released for distribution.
  • All KOMP targeting vectors received (in glycerol stocks) will be sent to CHORI for QC, archiving, and distribution. A small percentage of vectors will be re-sequenced and transformed. Once targeting vectors pass QC at CHORI, they will then be archived and released for distribution.
  • All KOMP mice, embryos, and sperm received from the mutagenesis teams will also be rederived and used to create a cryopreserved embryo and sperm archive which will subsequently be released for distribution.
  • All KOMP-related data received in association with products from the KOMP mutagenesis teams is screened for errors and scrubbed prior to posting on our website (www.komp.org) catalog, list of protocols, \“product detail files\” (PDF), etc.
The KOMP Repository is the sole archive and distribution repository for vectors, ES cells, and mice generated by the KOMP mutagenesis centers. Supported by an NIH cooperative agreement (U42 mechanism) to the University of California, Davis (UCD) and its subcontractor, Children's Hospital Oakland Research Institute (CHORI), the KOMP Repository ensures the preservation, protection, availability, and accessibility of KOMP resources for use by the scientific community. The repository is upon the closely linked infrastructure, technical support, and faculty expertise within the UCD Mouse Biology Program, Center for Comparative Medicine, the Schools of Medicine and Veterinary Medicine, and the CHORI-BACPAC Resource. UCD archives, maintains, and distribute ES cell clones, live mouse lines, and frozen embryos and sperm, while CHORI archives, maintains, and distribute all vectors.

The KOMP Repository Collection is located at the MMRRC at the University of California, Davis and Children’s Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at mmrrc@ucdavis.edu, US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors komporders@chori.org or +1-510-450-7917.