KOMP Knock Out Knockout Mouse Mice |
Industry Fees for KOMP Products and Services
The KOMP Repository was originally funded by a U42 grant from the National Institutes of Health (NIH)/National Center for Research Resources (NCRR) with a subcontract to Children’s Hospital Oakland Research Institute. While our NIH grant enabled us to establish the repository infrastructure, this grant ended in 2012, and fees have been modified for KOMP products and services to cover our operating costs.
The repository distributes KOMP vectors, ES cells, mice, and other materials, such as cryopreserved germplasm, DNA, etc. In addition, the repository also provides services to enhance the utility of these products, including ES cell microinjection, recombination, genetic analysis, pathogen screening, cryo-recovery, colony management, and other services.
Fees listed below are for For-Profit entities. For-profit entities are required to pay a twenty-five thousand dollars (US $25,000) per knockout Line of knockout Mice or twenty thousand dollars (US $20,000) per KO Line of ES Cells or Targeting Vectors for mutant products as per the MTA for KOMP Targeted Products (Vectors, ES cells, Mice, Germplasm), in addition to our distribution costs below. Additionally there are for-profit licensing requirements for wildtype cell lines (JM8, JM8.N4, JM8.F6, JM8A, JM8A3.N1, JM8A1.N3). Please contact us at firstname.lastname@example.org if you are looking to purchase these wild type cells.
Please, do not send license fee payment until you have placed your order online and have received an estimate and payment communication from our distribution representative.
We do offer additional breeding services; please inquire with us at email@example.com regarding fees.
When you are ready to place your request, you can do so online at www.komp.org. For an overview of the ordering process please see here. For additional services and fees, please contact us at firstname.lastname@example.org or 1-888-KOMP-MICE.
NOTE: Fees outlined here are subject to change without prior notice. Fees include administrative charges required by our institution for non-University of California customers. If you are with the University of California, please see here for UC pricing. An intercampus or campus recharge must be used for payment to receive UC pricing.
For domestic (within the USA) requests we accept payment via Purchase Order, Pre-Paid Check, Wire Transfer, and Credit Card. For international requests (outside the USA) we do require pre-payment via Wire Transfer, Pre-Paid Check, or Credit Card. Orders will not begin processing until we have received an online request, payment or payment details, and a completed Material Transfer Agreement. Pro-forma invoices can be provided upon request. Please, do not send payment or payment details until you have placed your order online and have received an estimate from our distribution representative.
Courier and Shipping Fees
Shipping and handling fees are not included in the above estimates. Handling fees will be included in your order estimate when the order is placed. In general, domestic shipments (U.S., not including Alaska, Hawaii, or insular territories) are subject to a $105.26 box and handling fee per ES Cell shipment, $ 176.47 handling plus $133.13 per crate for mouse shipments, and $154.80 handling fees for sperm or embryo shipments; all international shipments (plus Alaska, Hawaii and insular territories) are subject to a $292.57 box and handling fee per ES Cell shipment, $ 352.94 handling plus $266.26 crate fee, up to 2 crates, for mouse shipments, and $331.27 handling fees for sperm or embryo shipments. Special packaging requirements will incur extra fees. (Note: Mexico and Canada are international shipments requiring customs documentation.) Additionally, a License Processing Fee of $544.90 is added to your order total.
For most ES cell and vector shipments we can use Federal Express, and request that you provide us with your account number. There are some exceptions for certain countries that require extra documentation and handling, and for these we will recommend another specialized courier.
For cryo-materials, we can use Federal Express for domestic shipments, but for most international shipments we use one of our preferred international couriers, such as World Courier, BioCair or CryoPort, as customs requirements for biological materials are usually very strict.
For live animal shipments, we use one of our preferred live animal couriers, such as World Courier, Midnight Express (MNX), and Validated Courier. We prefer that you provide a courier account number so shipping fees will be billed directly to your institution from the courier. If you choose to use our account for shipping, the rate will be the shipping cost plus a 44.4% Administrative fee, which is required by our institution.
If you have a preferred courier, please let us know, as we will try to work with you on using that courier. However, there may be instances in which we strongly recommend against certain couriers for specific products based on our extensive experience in shipping mice and biologic materials.
Page last updated 11/20/2017
iA Fully Verified ES cell clone includes development of genotyping protocols, genetic verification at the 3' and 5' ends, vector copy number = 1, presence or loss of Y chromosome, chromosome count > or = 50% euploid, and mycoplasma and MPV tested. Type of genetic testing varies for CSD and Velocigene targets. Approximately 30% of KOMP clones tested fail genetic analysis and will require a new clone be selected for expansion and testing. Due to the average germline rate for KOMP targets, we recommend requesting and injecting 2 - 3 clones per targeted allele. All mutant ES cell lines are heterozygous for the mutation, meaning they carry one modified allele and one wild type allele. Please note, there is high incidence of mixed cell populations in the CSD targeted cell lines, and while our testing can detect this in most cases, it is not 100%, and there may be a very small percent of non or incorrectly targeted ES cells in the population, therefore we strongly recommend targeting verification of the derived mice with 5’ or 3’ LRPCR and/or Loss of Allele analysis.
iiBasic Microinjection provides injection of up to 3 ES Cell clones per gene, including injection of at least 28-32 embryos per clone, derivation of at least one litter containing a minimum of 8 pups, and housing until pups are 7 weeks of age. There are no guarantees for derivation of high percent chimeric mice with basic microinjection services.
iiiGuaranteed Microinjection Services includes microinjection of up to 3 ES cell clones per targeted allele (when available, ordered, and passing QC), and we guarantee to produce at least 2 appropriately sexed chimeras per targeted allele with greater than 50% ES cell derived coat color, or your money back from the injection service. The customer will not receive any ES cells as part of this package, but additional vials of cells can be ordered.
ivThis is an estimated fee for germline transmission testing, which includes mating of all high percentage chimeric males per clone, Project Management and monthly updates, per diem of breeder and non-breeder mice, colony husbandry, C57BL/6N female purchase, and genotyping of litters, including targeting verification on CSD allele targets. The actual fees depend on the fertility and fecundity of the chimeras and the number of litters generated and tested before heterozygous mice are identified. Fees may be higher or lower than estimated here. This is not a guaranteed service; fees are billed on services provided, including per diem, genotyping, project and colony management. Average germline success rates for the KOMP Project can be found at https://www.komp.org/gltrates.php.
vOne aliquot is one vial or straw of sperm with sufficient concentration to fertilize multiple clutches of oocytes, for derivation of at least one litter of mice if performed at the KOMP Repository. Please be aware that we cannot guarantee successful reanimation when recovery is performed outside the KOMP Repository.
The KOMP Repository is located at the University of California Davis and Children’s Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at email@example.com, US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors firstname.lastname@example.org or +1-510-450-7917.